Luke Lab
Research Publications Group Members Biography2024
Gameiro E, Juárez-Núñez KA, Fung JJ, Shankar S, Luke B and Khmelinskii A (2024) Genome-wide conditional degron libraries for functional genomics. bioRxiv , doi: https://doi.org/10.1101/2024.05.29.596381 Link
Siametis A*, Stratigi K*, Giamaki D, Chatzinikolaou G, Akalestou-Clocher A, Goulielmaki E, Luke B, Schumacher B and Garinis GA (2024) Transcription stress at telomeres leads to cytosolic DNA release and paracrine senescence. Nat Commun , 5:4061 (*indicates joint contribution) Link
2023
Nischwitz E*, Schoonenberg VAC*, Fradera-Sola A, Dejung M, Vydzhak O, Levin M, Luke B, Butter F# and Scheibe M# (2023) DNA damage repair proteins across the Tree of Life. iScience , 26:106778 Link
Pires V, Lohner N, Wagner T, Wagner C, Wilkens M, Hajikazemi M, Paeschke K, Butter F and Luke B (2023) RNA-DNA hybrids prevent resection at dysfunctional telomeres. Cell Rep , 42:112077 Link
Schindler N*, Tonn M*, Kellner V, Fung JJ, Lockhart A, Vydzhak O, Juretschke T, Möckel S, Beli P, Khmelinskii A and Luke B (2023) Genetic requirements for repair of lesions caused by single genomic ribonucleotides in S phase. Nat Commun , 14:1227 (* indicates joint contribution) Link
2022
Guintini L*, Paillé A*, Graf M, Luke B, Wellinger RJ# and Conconi A# (2022) Transcription of ncRNAs promotes repair of UV induced DNA lesions in Saccharomyces cerevisiae subtelomeres. PLOS Genet , 18:e1010167 (*indicates joint contribution, #indicates joint correspondence) Link
Kissling VM, Reginato G*, Bianco E*, Kasaciunaite K, Tilma J, Cereghetti G, Schindler N, Lee SS, Guérois R, Luke B, Seidel R, Cejka P# and Peter M# (2022) Mre11-Rad50 oligomerization promotes DNA double-strand break repair. Nat Commun , 13:2374 (*indicates joint contribution, #indicates joint correspondence) Link
Li L, Wang J, Yang Z, Zhao Y, Jiang H, Jiang L, Hou W, Ye R, He Q, Kupiec M, Luke B, Cao Q, Qi Z, Li Z and Lou H (2022) Metabolic remodeling maintains a reducing environment for rapid activation of the yeast DNA replication checkpoint. EMBO J , 41:e108290 Link
Misino S, Busch A, Wagner C, Bento F and Luke B (2022) TERRA increases at short telomeres in yeast survivors and regulates survivor associated senescence (SAS). Nucleic Acids Res , 50:12829–12843 Link
Pérez-Martínez L, Wagner T and Luke B (2022) Telomere interacting proteins and TERRA regulation. Front Genet , 13:872636 Link
Spada S, Luke B and Danckwardt S (2022) The bidirectional link between RNA cleavage and polyadenylation and genome stability: recent insights from a systematic screen. Front Genet , 13:854907 Link
Wagner CB and Luke B (2022) DNA–RNA hybrids at telomeres in budding yeast. Methods Mol Biol , 2528:145-157 Link
2021
Mosler T, Conte F, Longo GMC, Mikicic I, Kreim N, Möckel MM, Petrosino G, Flach J, Barau J, Luke B, Roukos V and Beli P (2021) R-loop proximity proteomics identifies a role of DDX41 in transcription-associated genomic instability. Nat Commun , 12:7314 Link
Wagner T, Pérez-Martínez L, Schellhaas R, Barrientos-Moreno M, Öztürk M, Prado F, Butter F and Luke B (2021) Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence. PLOS Genet , 16:e1008603 Link
2020
Niehrs C* and Luke B* (2020) Regulatory R-loops as facilitators of gene expression and genome stability. Nat Rev Mol Cell Biol, 21:167–178 (*indicates joint contribution) Link
Pérez-Martínez L, Öztürk M, Butter F# and Luke B# (2020) Npl3 stabilizes R-loops at telomeres to prevent accelerated replicative senescence. EMBO Rep, 21:e49087 (#indicates joint correspondance) Link
Vydzhak O, Luke B and Schindler N (2020) Non-coding RNAs at the eukaryotic rDNA locus: RNA-DNA hybrids and beyond. J Mol Biol, 432:4287–4304 Link
Vydzhak O*, Bender K*, Klermund J, Busch A, Reimann S and Luke B (2020) Checkpoint adaptation in recombination-deficient cells drives aneuploidy and resistance to genotoxic agents. DNA Repair, 95:102939 (*indicates joint contribution) Link
2019
Jolivet P, Serhal K, Graf M, Eberhard S, Xu Z, Luke B and Teixeira MT (2019) A subtelomeric region affects telomerase-negative replicative senescence in Saccharomyces cerevisiae. Sci Rep, 9:1845 Link
Kellner V# and Luke B# (2019) Molecular and physiological consequences of faulty eukaryotic ribonucleotide excision repair. EMBO J, 39:e102309 (#indicates joint correspondence) Link
Lockhart A, Pires VB, Bento F, Kellner V, Luke-Glaser S, Yakoub G, Ulrich HD and Luke B (2019) RNase H1 and H2 are differentially regulated to process RNA-DNA hybrids. Cell Rep, 29:2890–2900.e5 Link
Schmidt TT, Sharma S, Reyes GX, Kolodziejczak A, Wagner T, Luke B, Hofer A, Chabes A and Hombauer H (2019) Inactivation of folylpolyglutamate synthetase Met7 results in genome instability driven by an increased dUTP/dTTP ratio. Nucleic Acids Res, 48:264–277 Link
2018
Luke B (2018) Telomere regulation. Differentiation, 102:27–29 (guest editor of the issue) Link
Michelini F, Jalihal AP, Francia S, Meers C, Neeb ZT, Rossiello F, Gioia U, Aguado J, Jones-Weinert C, Luke B, Biamonti G, Nowacki M, Storici F, Carninci P, Walter NG and d’Adda di Fagagna F (2018) From “cellular” RNA to “smart” RNA: Multiple roles of RNA in genome stability and beyond. Chem Rev, 118:4365–4403 Link
Misino S, Bonetti D, Luke-Glaser S and Luke B (2018) Increased TERRA levels and RNase H sensitivity are conserved hallmarks of post-senescent survivors in budding yeast. Differentiation, 100:37–45 Link
2017
Graf M*, Bonetti D*, Lockhart A*, Serhal K, Kellner V, Maicher A, Jolivet P, Teixeira MT and Luke B (2017) Telomere length determines TERRA and R-loop regulation through the cell cycle. Cell, 170:72–85 (*indicates joint contribution) Link
Lafuente-Barquero J, Luke-Glaser S, Graf M, Silva S, Gómez-González B, Lockhart A, Lisby M, Aguilera A and Luke B (2017) The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. PLOS Genet, 13:e1007136 Link
2016
Buser R*, Kellner V*, Melnik A, Wilson-Zbinden C, Schellhaas R, Kastner L, Piwko W, Dees M, Picotti P, Maric M, Labib K, Luke B# and Peter M# (2016) The replisome-coupled E3 ubiquitin ligase Rtt101Mms22 counteracts Mrc1 function to tolerate genotoxic stress. PLOS Genet, 12:e1005843 (*indicates joint authorship, #indicates joint correspondence) Link
Dieckmann AK, Babin V, Harari Y, Eils R, König R, Luke B# and Kupiec M# (2016) Role of the ESCRT complexes in telomere biology. MBio, 7:e01793-16 (#indicates joint correspondence) Link
Poos AM, Maicher A, Dieckmann AK, Oswald M, Eils R, Kupiec M, Luke B and König R (2016) Mixed integer linear programming based machine learning approach identifies regulators of telomerase in yeast. Nucleic Acids Res, 44:e93 Link
Silva S, Altmannova V, Luke-Glaser S, Henriksen P, Gallina I, Yang X, Choudhary C, Luke B, Krejci L and Lisby M (2016) Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance. Genes Dev, 30:700–717 Link
Styles EB, Founk KJ, Zamparo LA, Sing TL, Altintas D, Ribeyre C, Ribaud V, Rougemont J, Mayhew D, Costanzo M, Usaj M, Verster AJ, Koch EN, Novarina D, Graf M, Luke B, Muzi-Falconi M, Myers CL, Mitra RD, Shore D, Brown GW, Zhang Z, Boone C and Andrews BJ (2016) Exploring quantitative yeast phenomics with single-cell analysis of DNA damage foci. Cell Syst, 3:264–277.e10 Link
2015
Feuerhahn S, Chen L, Luke B# and Porro A# (2015) No DDRama at chromosome ends: TRF2 takes centre stage. Trends Biochem Sci, 40:275–285 (#indicates joint correspondence) Link
Rippe K# and Luke B# (2015) TERRA and the state of the telomere. Nat Struct Mol Biol, 22:853–858 (#indicates joint correspondence) Link
Stockklausner C*, Raffel S*, Klermund J, Bandapalli OR, Beier F, Brümmendorf TH, Bürger F, Sauer SW, Hoffmann GF, Lorenz H, Tagliaferri L, Nowak D, Hofmann WK, Buergermeister R, Kerber C, Rausch T, Korbel JO, Luke B#, Trumpp A# and Kulozik AE# (2015) A novel autosomal recessive TERT T1129P mutation in a dyskeratosis congenita family leads to cellular senescence and loss of CD34+ hematopoietic stem cells not reversible by mTOR-inhibition. Aging, 7:911–927 (*indicates joint contribution, #indicates joint correspondence) Link
2014
Balk B, Dees M, Bender K and Luke B (2014) The differential processing of telomeres in response to increased telomeric transcription and RNA-DNA hybrid accumulation. RNA Biol, 11:95–100 Link
Klermund J, Bender K and Luke B (2014) High nutrient levels and TORC1 activity reduce cell viability following prolonged telomere dysfunction and cell cycle arrest. Cell Rep, 9:324–335 Link
Maicher A, Lockhart A and Luke B (2014) Breaking new ground: Digging into TERRA function. Biochim Biophys Acta - Gene Regul Mech, 1839:387–394 Link
2013
Balk B*, Maicher A*, Dees M, Klermund J, Luke-Glaser S, Bender K and Luke B (2013) Telomeric RNA-DNA hybrids affect telomere-length dynamics and senescence. Nat Struct Mol Biol, 20:1199–1205 (*indicates joint contribution) Link
2012
Luke-Glaser S and Luke B (2012) The Mph1 helicase can promote telomere uncapping and premature senescence in budding yeast. PLOS One, doi: 10.1371/journal.pone.0042028 Link
Luke-Glaser S, Poschke H and Luke B (2012) Getting in (and out of) the loop: Regulating higher order telomere structures. Front Oncol, 2:180 Link
Maicher A, Kastner L, Dees M and Luke B (2012) Deregulated telomere transcription causes replication-dependent telomere shortening and promotes cellular senescence. Nucleic Acids Res, 40:6649–6659 Link
Maicher A*, Kastner L* and Luke B (2012) Telomeres and disease: Enter TERRA. RNA Biol, 9:843–849 (*indicates joint contribution) Link
Poschke H*, Dees M*, Chang M, Amberkar S, Kaderali L, Rothstein R and Luke B (2012) Rif2 promotes a telomere fold-back structure through Rpd3L recruitment in budding yeast. PLOS Genet, 8:e1002960 (*indicates joint contribution) Link
2011
Ferreira HC, Luke B, Schober H, Kalck V, Lingner J and Gasser SM (2011) The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast. Nat Cell Biol, 13:867–874 Link
Iglesias N, Redon S, Pfeiffer V, Dees M, Lingner J# and Luke B# (2011) Subtelomeric repetitive elements determine TERRA regulation by Rap1/Rif and Rap1/Sir complexes in yeast. EMBO Rep, 12:587–593 (#indicates joint correspondence) Link
2010
Luke-Glaser S, Luke B, Grossi S and Constantinou A (2010) FANCM regulates DNA chain elongation and is stabilized by S-phase checkpoint signalling. EMBO J, 29:795–805 Link
2009
Chang M, Luke B, Kraft C, Li Z, Peter M, Lingner J and Rothstein R (2009) Telomerase is essential to alleviate Pif1-induced replication stress at telomeres. Genetics, 183:779–791 Link
Deplazes A, Möckli N, Luke B, Auerbach D and Peter M (2009) Yeast Uri1p promotes translation initiation and may provide a link to cotranslational quality control. EMBO J, 28:1429–1441 Link
Luke B and Lingner J (2009) TERRA: Telomeric repeat-containing RNA. EMBO J, 28:2503–2510 Link